Hello Group, I am trying to sort bacteria on a MoFlo with a 70 micron tip at 60psi. My problem is that when triggering on FS or SSC a large percentage of my cells fall within the "noise". If the bugs are labeled with a bright fluorophore, I can trigger on a fluorescent parameter with no problem. Since I need to keep FL1 free as my sorting parameter, I'd like to label all the bacteria with a dye that has adequate excitation at 488 and emission (red) that doesn't fall into my 530 FL1 filter. Does anyone in flow-land know of a dye that will label all (or at least viable) bacteria, has red emission, and is non-toxic to bacteria? Also, for you jet-in-air sorters.... what is the lower size limit in your hands of particle size that is distinguishable from noise on FS and SSC? I can see 0.9 micron beads fine triggering on FS, but the bacteria are more problematic. Thanks very much, Bill Throndset Genencor, International Palo Alto, CA -------------------------------------------------------------------------- This message (including any attachments) may contain information which is confidential or privileged. Use, dissemination, distribution, or reproduction of this message by unintended recipients is not authorized and may be unlawful. If you are not the intended recipient, please advise the sender immediately by reply e-mail and delete this message and any attachments without retaining a copy. --------------------------------------------------------------------------Received on Fri Mar 19 14:58:00 2004
This archive was generated by hypermail 2.1.8 : Sat Mar 27 2004 - 03:12:02 EST
