Re: Detection of Sepahrose beads by FCM

Sven,

Do you make your own beads?  You could add something to them to give them 
more scatter, such as an abundance of smaller beads.  This approach has 
been used to add more scatter signal to agarose microdroplets and agarose 
encapsulated cells.  In my experience, agarose drops didn't have much FSC, 
as you have recognized.  You could also try threshold on SSC instead.

Dave

David McFarland
GlaxoSmithKline




"Björnsson Sven G" <Sven.G.Bjornsson@skane.se>

18-Mar-2004 06:22

 
 

	To:	"Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu>

	cc: 
	Subject:	Detection of Sepahrose beads by FCM

Hello All,

I would like to study Heparin-binding proteins using Heparin-Sepaharose 
beads in FCM.
Problem is that i am not able to detect Sepharose CL-4 beads as FSC/SSC. 
These beads are
pretty big and I use a Coulter Epics witn the neutral density filter 
engaged to avoid
overloading the FS-detector. I still can´t see the beads. Is agarose beads 
too gelly to
scatter light? 


Sven Björnsson 
Cytometrilab
Labmedicin
S-205 02 Malmö
Sweden
Tel 040 33 83 37