RE: Is It possible to do GFP and YFP together

Hi Janet:
We have used a 510/21 BP for gfp, a 550/30 BP for yfp with a 525SP
dichroic to split the two, as recommended by Bill Telford. It works
reasonably well as long as there is a reasonable signal to measure.

Joanne Lannigan, MS
Director, Flow Cytometry Core Facility
University of Virginia
Jordan Hall, Room 7067
P.O. Box 800734
Charlottesville, VA 22908-0734
Office: 434-924-0274
Lab: 434-243-2695
Fax: 434-982-1071
email: joannelannigan@virginia.edu

> -----Original Message-----
> From: janet dow [mailto:jldow@unity.ncsu.edu]
> Sent: Tuesday, March 16, 2004 9:48 AM
> To: Cytometry Mailing List
> Subject: Is It possible to do GFP and YFP together
> 
> Dear Flowers:
> 
> I have a client who wants to try to sort GFP	and YFP containing
> cells from other populations.  His cells will be transfected with two
> separate plasmids, and they want the double positives as well as each
> single positive population.
> 
> I have a MoFlo with a 488nm, 635nm and UV laser.  Is it possible to
> configure the filters to do both colors and what would those
> configurations be???
> 
> As always thanks in advance for your information and support.
> 
> Janet Dow
> --
> Janet Dow
> Research Technician and Manager
> Flow Cytometry and Cell Sorting Facility
> North Carolina State College of Veterinary Medicine
> Room C-309
> Raleigh, NC 27606
> (919)513-6443