Volume of cells

Dear Flowers,

is there a possiblityy to measure the volume of cells by flow? The only thing I know is
to set a standard with different sort of beads by size to get different FSC values and
then calculate the appproximate volume of the cells by diameter. On the other hand I also
know that cells can expand and shrink depending on the medium the are stored in for
analysis and it also depends on the type of cells and how they pass the intercept of the
laser. 
Colleagues want to have an idea of the volume of cytoplasma of ficoll separated
mononuclear cells from	drug treated patients with regard of the amount of drugs retained
within the cells. 
Maybe someone has an idea.

All comments are welcome and thanks in advance.

Regards

Volker

Volker Eckstein PhD
Dept. of Internal Medicine V
Medical school of the university
University of Heidelberg
GERMANY
volker_eckstein@med.uni-heidelberg.de
Tel. +49 6221 56 37356
Fax. +49 6221 56 5775