Re: Need help finding antibodies directly conjugate to dyes for a violet laser.

Brent,

You can purchase custom conjugates of Pacific Blue from BD.  We have 
done this for CD45, CD38 and HLA-DR with excellent results.  AmCyan 
conjugates are also available as custom conjugates from BD and can be 
used as a second fluorochrome in combination with Pacific Blue, 
although there will be additional compensation with FITC..

Brent Wood
Director, Hematopathology Laboratory
Department of Laboratory Medicine
University of Washington, Seattle, WA, USA
E-mail: woodbl@u.washington.edu  Phone:(206) 598-6199  FAX: (206) 
598-6189
On Mar 10, 2004, at 2:40 PM, Fischer, Randy (NIH/NIAMS) wrote:

> Brent,
>
> They are not currently available, you need to use the Zenon kits or
> succinimidyl esters from Molecular Probes for the 405/Pacific Blue, 
> and a
> biotin:Streptavidin for the 430 channel.  Alternatively, Mario 
> Roederer may
> have some other info about better options.
>
> And as always, being a government employee, it is my duty (and the 
> truth) to
> say I get nothing from Molecular Probes for my statements here.
>
> Randy T. Fischer
> NIH/NIAMS
> Building 10, Room 6D50
> 9000 Rockville Pike
> Bethesda, MD 20892
> (301) 594-3537
> fischer1@mail.nih.gov
>
>> ----------
>> From:	Brent.Palmer@UCHSC.edu
>> Sent:	Tuesday, March 9, 2004 1:07 PM
>> To:	Cytometry Mailing List
>> Subject:	Need help finding antibodies directly conjugate to dyes for
>> a violet laser.
>>
>>
>> We recently purchase a BD Aria and we are trying to locate any
>> commercially available anti-human antibodies that can be used with the
>> violet laser (407 nm).  More specifically are there any companies that
>> make directly conjugate anti-human CD3 or CD4 mABs that are 
>> conjugated to
>> cascade blue, alexa flour 405, pacific blue, DAPI or some equivalent?
>>
>> Thanks in advance.
>>
>> Brent Palmer PH.D
>> Division of Infectious Diseases and Clinical Immunology
>> University of Colorado Health Sciences Center
>> Denver, CO.
>> 303-315-0762
>>
>>