Hi - You can commercially do custom conjugates to some of the fluorchromes you mentioned through BD (and perhaps other companies as well - anyone have information on this?). Caltag is selling rat anti mouse Ab directly conjugated to Alexa 405. There has been enough talk and testing of these things that I assume the market will open up soon. Marty Marty Bigos Director, Flow Core Gladstone Institute of Virology and Immunology Building 3 SFGH Rm 509 415-695-3832 >Brent, > >They are not currently available, you need to use the Zenon kits or >succinimidyl esters from Molecular Probes for the 405/Pacific Blue, and a >biotin:Streptavidin for the 430 channel. Alternatively, Mario Roederer may >have some other info about better options. > >And as always, being a government employee, it is my duty (and the truth) to >say I get nothing from Molecular Probes for my statements here. > >Randy T. Fischer >NIH/NIAMS >Building 10, Room 6D50 >9000 Rockville Pike >Bethesda, MD 20892 >(301) 594-3537 >fischer1@mail.nih.gov > >> ---------- >> From: Brent.Palmer@UCHSC.edu >> Sent: Tuesday, March 9, 2004 1:07 PM >> To: Cytometry Mailing List >> Subject: Need help finding antibodies directly conjugate to dyes for >> a violet laser. >> >> >> We recently purchase a BD Aria and we are trying to locate any >> commercially available anti-human antibodies that can be used with the >> violet laser (407 nm). More specifically are there any companies that >> make directly conjugate anti-human CD3 or CD4 mABs that are conjugated to >> cascade blue, alexa flour 405, pacific blue, DAPI or some equivalent? >> >> Thanks in advance. >> >> Brent Palmer PH.D >> Division of Infectious Diseases and Clinical Immunology >> University of Colorado Health Sciences Center >> Denver, CO. >> 303-315-0762 >> >> --Received on Fri Mar 12 12:58:00 2004
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