Hi - On the DiVa software, linear or log display is just that, a display option. All data is collected (or scaled to) 18 bit linear (compensated data may be broader since arithmetic operations are involved). The data has the same values no matter how you display it, 2.6 to 262K. If you display it as four decades log, the lower decade (2.6 to 26) will be compressed into the left edge of the scale. Statistics in DiVa are computed on the full data range and are independent of the display option chosen. Thus, even though you only display four decades, the mean of autofluorescent cells can be "below the bottom" i.e. less than 26. Choosing linear or log as a display option does not change this. Hope this is clearer than mud! Marty >Hi, >Would anyone who is using the BD DiVa option have worked out how to >accurately determine a channel value on a log scale? It was easy to do in >Analogue because it was built into the program. It is also easy to do in a >Digital Linear scale. Any thoughts would be very helpful. >Thank you >Leonie >Haematology >POWH >Sydney >Australia > > >Attachment converted: Asimov:InterScan_Disclaimer.txt (TEXT/ttxt) (0014C701) -- Marty Bigos Director, Flow Cytometry Core Laboratory Gladstone Institute of Virology and Immunology Mail: Box 419100 San Francisco, CA 94141 Packages: 365 Vermont Street San Francisco, CA 94103 Direct Delivery (FedEX, UPS): Gladstone Institute San Francisco General Hospital Building 3 Rm 509 1001 Potrero Avenue San Francisco, CA 94110 (tel) 415-695-3832 (fax) 415-826-8449 mbigos@gladstone.ucsf.edu http://www.gladstone.ucsf.eduReceived on Wed Mar 10 14:58:00 2004
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