dear Flow gurus, Being a bit of a ninny w.r.t flowcytometry ,need some help. I am trying to look at up/down regulation of certain surface markers in transiently trasfected eGFP+ cells (FACScalibur).I use class-I staining (on FITC on the same cells) for setting FITC/PE compensations , the problem is that the eGFP+ cells (but not the FITC+ controls) look like theyve been over compensated, everything goes and sticks to the axis. the proportion of GFP+s cells is so low that i am not sure i shd rely on them to actually set compensations..one would expect FITC and GFP to behave similarly wudnt one? Please enlighten rama ________________________________________________________________________ Yahoo! Messenger - Communicate instantly..."Ping" your friends today! Download Messenger Now http://uk.messenger.yahoo.com/download/index.htmlReceived on Mon Mar 8 12:38:00 2004
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