Hi Amir, There's a Cy-dye from Amersham that fluoresces after a drop in pH, such as occurs after protein internalization into lysosomes. They sell the usual easy-labelling kits, I think. I don't know of any references in flow cytometry, nor do I know the excitation/emission wavelenghts by heart, but it may just be the thing for you. Good luck, Guy Guy Hermans, PhD Ablynx N.V. Technologiepark 4 9052 Zwijnaarde Belgium Phone : +32 9 261 06 23 Fax : +32 9 261 06 27 _______________________________________________ -----Original Message----- From: Amir Ghaemmaghami [mailto:Amg@nottingham.ac.uk] Sent: Thursday, March 04, 2004 10:01 AM To: cyto-inbox Subject: Dear all, Dear all, We are trying to study protein uptake by DC using flowcytometry. We have a 25KD protein which we want to label with a fluorochrome. We are not quite sure what would be the best approach. Any suggestions (protocols) are very much appreciated. Thank you ++++++++++++++++++++++++++++++++++ A. M. Ghaemmaghami, MD PhD Department of Immunology A Floor, West Block Queen's Medical Centre University of Nottingham Nottingham NG7 2UH UK Tel:+44(115)9709162 or 9249924 ext 43293 Fax:+44(115)9709125 Email: amg@nott.ac.uk ----------------------------------------------------------------------- THIS E-MAIL MESSAGE IS INTENDED ONLY FOR THE USE OF THE INDIVIDUAL OR ENTITY TO WHICH IT IS ADDRESSED AND MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE. If the reader of this E-mail message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately at ablynx@ablynx.com. Thank you for your co-operation. -----------------------------------------------------------------------Received on Fri Mar 5 13:18:00 2004
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