Dear All, We want to quantify the amount of endothelial cells (big cells > 10 um) in whole blood by using Flow count flourosheres beads. Does somebody have experience with these kind of experiments. We did some preliminary experiment in which we saw that the detection was linear had high amount of cells, but at low amount of cells it seems to me that the small particles of the Flow count flourosheres beads are causing troubles (we have to count more then 2 million cells to get enough endothelial cells (+/- 200 to analyse). Do I have to use bigger beads or more beads. Thank you very much in advance for your answers. Erik Mul, ing Sanquin Research at CLB Dept. of KVI Plesmanlaan 125 1066 CX AMSTERDAM The Netherlands phone: +31-205123435 fax: +31-205123310 email: e.mul@sanquin.nlReceived on Thu Mar 4 13:38:00 2004
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