Dear Kathleen, Simple answer - Yes! That's the reason you need so much compensation to get the PE-Cy5 signal out of the APC channel in an analyser such as a FACScalibur. In our FACStar Plus sorter, the direct Cy5 signal excited by the 600nm dye laser is only slightly less than the PE-Cy5 signal excited by 488. Cy5 has an absorption max of 650nm, so excitation at 633nm should be much more efficient than 600nm. Happy flowing and glowing! Joseph. At 10:13 PM 2/03/2004, Kathleen Marienfeld wrote: >Dear Flowers, >only one simple question: >Is it possible to detect a PE-Cy5-conjugate (Cy-Chrome) like Cy5 >just by exciting it at 633 nm?! >Thanks! >Kathleen -- Joseph Webster, Flow Cytometry Facility Centenary Institute, Sydney, AUSTRALIA. Phone +61-2-9565-6110Received on Wed Mar 3 12:38:00 2004
This archive was generated by hypermail 2.1.8 : Thu Mar 04 2004 - 03:12:02 EST
