Re: cell cycle analysis in 96 well format

In response to question #2...
Pall Corporation makes 96-well filter plates with 54 um screen.  The
catalog number for a
10 pak is PN 5049.  They work really well.

http://www.labfilters.com/catalog/00004280.asp

Good Luck,
Christie Doxsee
Immunologist
Drug Discovery, Pharmacology
Pharmaceutical Division
3M Center, Bldg. 270-2S-06
St. Paul, MN 55144

651-733-4906
fax: 651-737-5886
cdoxsee@mmm.com


|---------+----------------------------->
|	  |	      "Albert Tai"	|
|	  |	      <acktai@exelixis.c|
|	  |	      om>		|
|	  |				|
|	  |	      02/25/2004 03:47	|
|	  |	      PM		|
|	  |				|
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  |	 To:	   Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>		 
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  |	 Subject:  cell cycle analysis in 96 well format				 
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Hi flow cytometrists,

 I recently encountered some problems with passing PI stained cells
(previously fixed with ethanol) through the BD multi-well autosampler in 96
well format.		 Initially, the first few wells gave decent DNA
cell cycle profile.  Then the acquistion rate became to slow down
significantly as the cells became clumpy.

I would like to address the issue with the following questions and your
advices and opinion are very much appreciated.

1.  Is there any protocol that use PI with low concentration of detergent
that will give acceptable CV for cell cycle analysis while keeping the
cells from clumping ?

2.  Any 96 well format cell strainer available commercially ?

3.  Any other DNA dye for cell cycle analysis less 'sticky' than propidium
iodide ?

Thanks very much in advance for your help.

Albert Tai
Signal Transduction Research
Exelixis, Inc.
P.O. Box 511
170 Harbor Way
So San Francisco, CA 94083
(650)837-7228 (direct)
(650)837-7220 (fax)
e-mail:  acktai@exelixis.com