In a 1987 article "Analysis of T cell subsets in normal adults. comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry by P Renzi & L Ginns in Journal of Immunological Methods, it was found that, in normal individuals, ficoll separation of lymphocytes selectively decreases the CD8 subset compared to whole blood analysis on a buffy coat in all 32 individuals tested. The average CD4/CD8 ratio was 1.8 in the buffy coat and 2.8 in the ficolled specimen. We have seen results here that are not too different. Does anyone have any more recent information, experiences, or fixes they would like to share on this issue? Thanks. Marty -- Marty Bigos Director, Flow Cytometry Core Laboratory Gladstone Institute of Virology and Immunology Mail: Box 419100 San Francisco, CA 94141 Packages: 365 Vermont Street San Francisco, CA 94103 Direct Delivery (FedEX, UPS): Gladstone Institute San Francisco General Hospital Building 3 Rm 509 1001 Potrero Avenue San Francisco, CA 94110 (tel) 415-695-3832 (fax) 415-826-8449 mbigos@gladstone.ucsf.edu http://www.gladstone.ucsf.eduReceived on Wed Feb 25 12:18:00 2004
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