Monocytes /macrophages when phagocytize apoptotic bodies become positive for binding annexin V (Marquet et all., Nature, Cell Biol. 1999;1:454-459). Most likely the plasma membrane of the ingested apoptotic bodies fuses with the macrophage plasma membrane triggering externalization of phosphatidylserine. I am not surprized that Alice's monocytes appear happy because they just gulped a tasty bit of apoptotic body and are not hungry anymore. Zbigniew Darzynkiewicz, M.D., Ph.D. Brander Cancer Research Institute New York Medical College 19 Bradhurst Ave. Hawthorne, NY 10532 tel: 914-347-2801 fax: 914-347-2804 http://www.geocities.com/z_darzynkiewicz > -----Original Message----- > From: Alice L. Givan [SMTP:Alice.L.Givan@Dartmouth.EDU] > Sent: Thursday, February 12, 2004 3:57 PM > To: Cytometry Mailing List > Subject: annexin V on monocytes > > Hello Flowers, > Using the annexin V/PI assay for apoptosis, a group has found high positivity for > external annexin V on supposedly happy human monocytes. These monocytes are negative > for PI. Has anyone looked at apoptosis in monocytes by this assay? > > If you reply directly to me, I will summarize answers back to the network. > > Alice > > Alice L. Givan, Director > Englert Cell Analysis Laboratory > of the Norris Cotton Cancer Center > Dartmouth Medical School > Lebanon, NH 03756 USA > tel 603-650-7661 > fax 603-6540-6130 > givan@dartmouth.edu > www.dartmouth.edu/~celllab >Received on Mon Feb 16 15:58:00 2004
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