Try freezing a small aliquot for a few days , then thaw and compare to unfrozen conjugate -----Original Message----- From: Regis Josien To: cyto-inbox Sent: 2/13/04 3:13 AM Subject: which conjugates can be frozen? Dear flowers, I am posting again this message. I turned out that a lot of people are interested by the response to this question about the possibility to freeze conjugated Abs. I appreciate your suggestion. I have a number of custom conjugates (PE-Cy7, APC-Cy7, PerCP-Cy5.5, Alexa488 or 647) in quite large quantities and long time storage is a problem. I heard it is not recommended to freeze such conjugates but I wonder if anyone has experience in freezing directly conjugated mAb. I use to freeze FITC conjugates with no loss in activity but what about: PE PerCP PerCP-Cy5 or 5.5 PE-Cy7 APC APC-Cy7 Alexa conjugates. Thanks. -- Regis Josien, MD, Ph.D Institut de Transplantation et de Recherche en Transplantation INSERM U437 30 bd Jean Monnet 44093 Nantes Cedex 1 France tel : +33-240-087-413 sec: +33-240-087-410 fax: +33-240-087-411 email : rjosien@nantes.inserm.fr rjosien@chu-nantes.frReceived on Mon Feb 16 15:38:01 2004
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