Alice: yes, we have done monocytes with annexin V and we get a very high percentage of positive cells. The monocytes have some phagocytic activity and I believe that they phagocytize the annexin V. Therefore, I don't think that you can use monocytes for this assay. I would fix the cells and use a stain for active caspase 3 (Cell Signaling makes a great antibody). Richard S. Hotchkiss MD Professor of Anesthesiology, Medicine, Surgery, and Molecular Biology and Pharmacology Washington University School of Medicine phone 314-3628552 fax 314-3628571Received on Mon Feb 16 13:58:00 2004
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