Hi All, I'm interested in the detection of intracellular NAD(P)H by flow, but need some advice. NAD(P)H is a very poor fluorophore with a quantum yield of about 2.8%. It is easily seen under UV excitation with a DAPI filter by fluorescence microscopy. However, to state the obvious, cells are not in the laser for seconds in a flow cytometer like they are on a microscope. David Piston at Vanderbilt has published several very nice papers on the use of two photon excitation to visualize NAD(P)H by fluorescence microscopy. Does anyone have experience with two-photon excitation lasers and flow cytometers? Does anyone have experience trying to visualize NAD(P)H either with a standard UV laser or two-photon titanium saphire laser? Thanks for your help!! Matt Matthew S. Hanson, Ph.D. Asst. Scientist Dept. of Surgery (H5/301) UW-Madison Hospitals & Clinics 600 Highland Avenue Madison, WI 53792-3236 Tel: 608-262-9602 Fax: 608-265-9144 Matthew S. Hanson, Ph.D. Asst. Scientist Dept. of Surgery (H5/301) UW-Madison Hospitals & Clinics 600 Highland Avenue Madison, WI 53792-3236 Tel: 608-262-9602 Fax: 608-265-9144Received on Thu Feb 5 16:58:00 2004
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