Hi Flow Folks I have an investigator who is looking to develop an assay to relate level of persistent fetal derived mononuclear cells in female stem cell donors to engraftment. this may either be mature or immature cells, but she would like to identify these cells by detecting H-Y antigen encoded proteins or the Y chromosome itself...does anyone have any suggestions here? Are there flow assays that might do this...I could think that flow fishing might work, but I do not know of any particular Y antigen specific proteins that might be surface expressed...except for the remote control gene... Any ideas? Thanks, Jonni -- Jonni S. Moore, Ph.D. Associate Professor of Pathology and Laboratory Medicine Director of the Biomolecular and Cellular Resource Center Director of Clinical Flow Cytometry Director, Flow Cytometry and Cell Sorting Shared Resource Abramson Cancer Center University of Pennsylvania School of Medicine 203 John Morgan Bldg. Philadelphia, PA 19104-6082 Phone: 215-898-6853 FAX: 215-898-0734Received on Thu Feb 5 16:18:00 2004
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