Dear Flowers, I have an investigator who wishes to do four colors, as a rule that would be no big deal. Here is the sticker, and not wishing to insult anyone, but I will give the ems, this want to use 7-AAD as one of the stains for viability. Fitc: 519 PE: 578 APC: 660 7-AAD: 647 or would I be better using Ethidium Bromide: 620 If any flowers out there have and opinion on stains and proper filters it would be immensely appreciated. I will be doing the analysis on a LSR-1 [488, HeNe, & UV] to see if it is feasible to sort. I will sort on a FACSVantage SE [air cooled Argon, HeNe, & a water cooled UV]. I will probably use CellQuest since I am using it on the LSR-1, but if there is too much spectral overlap, I will use DiVa. With no exposures[ref. Super Bowl LOL]. Thanks in advance to all. Howard Howard S. Mostowski HHS/FDA/DCGT Bldg.29A/ Rm 3C10 9000 Rockville Pike Bethesda, Md. USA 20894 [301] 827-0704Received on Wed Feb 4 13:58:00 2004
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