Dr. Quarta, We use aminostilbamidine methanesulfonate (ASBMS) almost exclusively on our instruments with UV excitation. Once upon a time we used to purchase hydroxySBMS. Well, at least we thought we did. It turned out that our lot of HSBMS purchased from Molecular Probes was mislabeled and was in fact ASBMS. To be certain of what I had and what I wanted, I ordered new lots of each. HSBMS worked OK but ASBMS was superior. We make a 10mM stock in DMSO and use it 1:1000. I compared results between PI, 7AAD, TOPRO-3 and ASBMS on a BD LSR and got virtually identical results. The staining pattern is similar to that of PI on the LSR (325nm excitation) with a good two logs of separation between live and dead. The separation isn't as good on the FACSDiVa sorter (350nm excitation) but good enough. I suspect it has more to do with the optics than the laser wavelength. Dave David McFarland GlaxoSmithKline ----- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 28-Jan-2004 09:26 ----- mattiaqu@tin.it 24-Jan-2004 07:22 To: "Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu> cc: Subject: PerdueCytometry: Fluoro Gold viability stain Dear all, does anybody has experience in evaluating cells viability with FluoroGold (hydroxystilbamidine methanesulfonate)? Any information and advise would be appreciated. Best regards. MATTIA QUARTA M.D. Department of Medical and Surgical Sciencies University of Padua Via Giustiniani 2 35100, PADOVA Italy Phone: +39-049-8211873 fax: +39-049-8211884Received on Fri Jan 30 22:58:00 2004
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