Re: Flow Safety Hood

From: R. Thomas Leonard <rtl5p@virginia.edu>
Date: Fri Jan 30 2004 - 14:18:36 EST
It's clearly a good practice to conduct HIV+ sample prep in a hood (i.e. 
Biological Safety Cabinet).

One regulation (i.e. law) that might apply in this case would be found 
in the U.S. Bloodborne Pathogen Standard (29 CFR 1910.1030) 
<http://www.osha.gov/pls/oshaweb/owadisp.show_document?p_table=STANDARDS&p_id=10051>. 
Your situation may be subject to interpretation, so I would encourage 
you to consult with your institution's biosafety officer to explore the 
applicability of the following sections of the standard:

HIV and HBV Research Laboratories and Production Facilities.

1910.1030(e)(1)

This paragraph applies to research laboratories and production 
facilities engaged in the culture, production, concentration, 
experimentation, and manipulation of HIV and HBV. It does not apply to 
clinical or diagnostic laboratories engaged solely in the analysis of 
blood, tissues, or organs. These requirements apply in addition to the 
other requirements of the standard.

1910.1030(e)(2)(ii)(E)

All activities involving other potentially infectious materials shall be 
conducted in biological safety cabinets or other physical-containment 
devices within the containment module. No work with these other 
potentially infectious materials shall be conducted on the open bench.

Regards, Tom

Abel, Robert wrote:

> Dear Flow-ers,
> In a clinical setting, for CD4 enumeration (HIV+) and LLP (BM, Nodes, 
> FNA) does anyone know if there are regulations requiring the use of a 
> hood for sample prep or is it just Good Lab Practice?
>
> Thanx for your input,
> Bob Abel
>
> Robert F. Abel
> Technical Specialist
> Christiana Care Health Services
> RAbel@christianacare.org
> (302) 733-3715
>
-- 

_________________________________
R. Thomas Leonard, PhD, CSP, CBSP
Biosafety Officer
University of Virginia
Office of Environmental Health and Safety
P.O. Box 400322
Charlottesville, VA 22904-4921

Phone: (434) 982-4928
Fax: (434) 982-4921
tleonard@virginia.edu
Received on Fri Jan 30 22:27:18 2004

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