Re: rat neut's mono's

Denise,

I have a  user routinely working with rat granulocytes and
monocytes/macrophages, usually in bronchial alveolar lavage (BAL) fluid, but
also sometimes in spleen.  Well defined surface antigens are limited for
these populations in the rat.  This user has settled on using RP-1 to
delineate granulocytes and 1C7 to get the "mononuclear phagocytes".  Both of
these were purchased from Pharmingen.  This combination seems to do a good
job separating out two very distinct populations based on their phagocytic
activity, however the RP-1 antigen has not been extensively described.	You
might consider one of the HIS-xx or ED-x monoclonal antibodies out of the
Netherlands in place of the 1C7, although we had trouble finding the ones we
wanted in the fluorochrome conjugates we needed.  I know from personal
experience that they are generally "good stainers" in tissue sections and
the few times I've used them for flow.	Both the  HIS and ED monoclonals are
available through Serotec.

FYI - If you're using BAL, be prepared.  Rat BAL cells have a VERY high
level of background autofluorescence.

Greg Perry
Creighton University




From: "Manfra, Denise" <denise.manfra@spcorp.com>
Date: Thu, 22 Jan 2004 10:31:46 -0500
To: cyto-inbox
Subject: rat neut's mono's



I am starting to work with rats and would like to do some Flow analysis.
Initially I want to look at neutrophils and monocytes/macrophages. Does
anyone have experience with staining for these cell types in rat blood,
tissue homogenates, or fluid exudates? I am searching for the best surface
antigens to stain for on these cells.

Thanks, 

Denise 

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