Hi Calin, Have you stained with gamma-delta TCR? There is a subset of T cells with gamma-delta that is increased in HIV patients that are also CD4-CD8-. Derya ------------------------------------------------- Derya Unutmaz, M.D. Assistant Professor of Microbiology and Immunology Vanderbilt University School of Medicine, 21st avenue South Medical Center North, Room: AA-5206 Nashville, TN 37232-2363 615-322 1435 (office) 615-322 1436 (lab) 615-294 3894 (cell phone) 615-343 7392 (fax) Emails: Derya@mac.com Derya.Unutmaz@vanderbilt.edu Lab email: retrovirus@mac.com ------------------- Hi! We are consistently finding a double negative (i.e., CD4-CD8-) population of T cells in most of our cases of pediatric AIDS and we have some difficulty in explaining the origin and nature of this population. In certain cases these cells can make up to 7-8% of the total number of CD3 cells from the peripheral blood. They seem to be some large lymphocytes expressing high levels of cell surface CD3. We have invariably seen this population using CD4/CD8 antibodies from different sources, so a staining artifact would be unlikely. Any input would be appreciated! Thanks a lot for your help, Calin.Received on Thu Jan 15 17:58:00 2004
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