Experience with G0/G1 in live cells with flow

From: Bushnell, Timothy <Timothy_Bushnell@URMC.Rochester.edu>
Date: Thu Jan 15 2004 - 11:56:17 EST
Hello all:

I have a user who wishes to look at G0 and G1 populations in live cells.  I
am wondering if anyone has used the Pyronin Y vs Hoechst in live cells and
if so, could they share the protocol.  Especially what pitfalls I need to be
aware of.

Thanks
Tim

Timothy Bushnell, Ph.D.
University of Rochester Center for Human Genetics and Molecular Pediatric
Diseases
Flow Cytometry Facility
1-11314



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