RE Aria ideal max. sort rate w/ viable cells

I am certainly no apologist for BD but my sense of fair play urges me to write. The
"white" technical paper for the Aria on the BD site
(http://www.bdbiosciences.com/immunocytometry_systems/products/display_product.php?keyID=
53) says that the maximum acqusition rate is 70 000 cells per sec (8 parameters 12
compensated pairs) but the average threshold rate when sorting is a more modest 25 000
cells per sec (70psi, 90kHz, 4 way sort). This could be a source of confusion.

All the best




	Hi Michele,


	Well if you throw in a couple of extra PMT's to process plus some compensation
you be able should get close to 20 000 events/sec. I figure that the marketing people at
BD should be ashamed of themselves for advertising such a blatantly wrong/unrealistic
number. Has anyone been able to get higher because I'd like to know under what conditions
and what their yield/purity was?


	Robert Sexton 
	Responsable Service Cytometry Facility Manager 
	Institut de recherche en immunovirologie 
	et en cancérologie (IRIC) 
	Université de Montréal 
	514 343 6111 ext 8094 
	mailto: robert.sexton@umontreal.ca 

	-----Original Message----- 
	From: Michele Black [mailto:mblack2@u.washington.edu] 
	Sent: Fri 09/01/2004 7:05 PM 
	To: Cytometry Mailing List 
	Cc: 
	Subject: Aria ideal max. sort rate w/ viable cells
	Hello All,
	Does anyone know the maximum sort rate on the Aria to recover viable cells (
Lymphocytes for example)? I know BD say 70000 events/ sec but this is not for viable
cells.
	I can sort up to 10000cells/ sec on a Vantage, is the Aria significantly higher? 
	Thanks in advance.
	Michele Black
	Director, Cell Analysis Facility
	Dept. of Immunology, Box 357650
	University of Washington
	Seattle, Wa 98195
	Phone: (206)685-3014
	 Fax: (206)543-1013
	 mblack2@u.washington.edu <mailto:mblack2@u.washington.edu> 



Ian Titley PhD
Leukaemia Research Fund Centre
at the Institute of Cancer Research
237 Fulham Road
London UK SW3 6JB

Tel +44 (0)20 7352 8133
Fax +44 (0)20 7352 3299