Stacy: What are you thresholding on? You should be thresholding on the fluorescence parameter of your DNA dye. Joanne Lannigan, MS Director, Flow Cytometry Core Facility University of Virginia Jordan Hall, Room 7067 P.O. Box 800734 Charlottesville, VA 22908-0734 Office: 434-924-0274 Lab: 434-243-2695 Fax: 434-982-1071 email: joannelannigan@virginia.edu > -----Original Message----- > From: Powell, Stacy [mailto:powell@sri.org] > Sent: Friday, January 09, 2004 3:24 PM > To: Cytometry Mailing List > Subject: Cell Cycle Analysis > > Hi Everyone! > > I was wondering if anyone has any ideas to why I am getting so much > "noise" > or background on some cell cycle samples that I am running. I have tried > adjusting the volts and gains and the auxillary channel ( I have a Coulter > XL) and there is so much background that I cannot get anything that looks > like a diploid ( or any ploidy). Could this possibly have something to do > with the alignment of the Cytometer? Any thoughts would be appreciated. > > Sincerely, > > Stacy Powell > Cell Biology/Immunology Dept. > Southern Research Institute > 2000 Ninth Avenue South > Birmingham, AL 35205 > 205-581-2243 > powell@sri.orgReceived on Tue Jan 13 14:58:00 2004
This archive was generated by hypermail 2.1.8 : Thu Jan 22 2004 - 12:12:47 EST
