RE: ssc/CD45 mouse bone marrow

Hi

In mouse bone marrow, we tend to use lineage-specific reagents, but CD45 in mouse bone
marrow looks pretty similar to human (based on my quick look in the literature for human,
I dont have first-hand experience).

I could send you data - if you think it would help you - that shows that all the mature B
cells in mouse bone marrow are CD45-bright and the  majority of CD45-bright low scatter
cells are B cells.  Is this what you are after ?

best regards,
Rachel
=======================================================
Rachel M. Gerstein, Ph.D.
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX)


> ----------
> From: 	mattiaqu@tin.it
> Sent: 	Wednesday, January 7, 2004 1:13 PM
> To:	Cytometry Mailing List
> Subject:	ssc/CD45 mouse bone marrow
> 
> Hi flowers,
>	       I've been looking extensively for references about ssc versus
> CD45 plots of mice bone marrow but could not find any.
> Does anybody can help with that? 
> Does anybody has experience with CD45 staning of mice bone marrow to differentiate
> hematopoietic populations?
> 
> I did some tests using a CD45.2 antibody from BD Pharmingen on Balb/c bone
> marrow but I cannot appreciate any real "gradient" of CD45 log intensity
> in order to differentiate lymphocytes from monocytes and granulocytes. Should
> it work like in humans bone marrow samples?
> 
> Any help would be appreciated,
> 
> Thanks
> 
> 
> 
> MATTIA QUARTA M.D. 
> Department of Medical and Surgical Sciencies
> University of Padua
> Via Giustiniani 2
> 35100, PADOVA
> Italy
> Phone: +39-049-8211873
> fax: +39-049-8211884
> 
> 
>