FW: CD antigen question

Happy New Year to all,

A question from one of my flow users.  Please answer Wayne directly at
WDyer@arcbs.redcross.org.au

Thanks

Jenny

Jenny Bryant
Flow Cytometry 
Australian Red Cross Blood Service 
153 Clarence St
Sydney
NSW  2000
Australia



> -----Original Message-----
> From: Dyer, Wayne (NSW) 
> Sent: Thursday, 8 January 2004 10:04 AM
> To:	Bryant, Jenny (NSW)
> Subject:	CD antigen question
> 
> Hi Jenny. Please post this to the flow group for me.
> Thanks,  
> Wayne.
> 
> I wish to compile a list of immunologically relevant CD markers that would
> show detectable changes in expression levels after exposure/culture with
> live viruses. This will be used to detect changes in cultured bulk
> leukocytes and PBMC after in-vitro viral exposure, as well as in
> leukocytes isolated from donors with concurrent viral infection. The aim
> is to use this info to construct a CD antibody protein array to examine
> changes in CD antigen expression (up or down regulation) that may
> specifically signify exposure to a particular group of viruses. Therefore,
> these CD markers need to be expressed in sufficient density in order to
> capture leukocytes to the solid phase bound antibodies. Once bound and
> fixed, secondary staining and confocal microscopy can then determine the
> predominant composition of cells at each CD spot (ie, CD4 and CD8 T cells,
> and very importantly, NK and other cells of the early innate response to
> infection).
> We have used this technology to rapidly phenotype diverse leukemia cell
> populations:
> (Belov L, de la Vega L, dos Remedios CG, Mulligan SP, Christopherson RI.
> Immunophenotyping of leukemias using a cluster of differentiation antibody
> array. Cancer Res (2001) 61: 4483-4489.)
> 
> We now wish to modify our array for additional applications, including the
> viral exposure study described above, which will be performed by my
> Honours student during 2004. In order to minimise resource expenditure
> through purchase on unnecessary antibodies, I seek your combined expertise
> on this matter. Any suggestions will be gratefully received.
> 
> Kind regards,
> 
> 
> Wayne B. Dyer, PhD
> Viral Immunology Lab, Tissue Typing Dept.
> ARCBS, 153 Clarence Street, Sydney, NSW 2000, Australia
> ph:	 +61 2 9229 4557
> fax:	 +61 2 9229 4521
> mob: +61 408 039 329
> 
> 
> 
> 
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