2003 A Review from the Purdue Cytometry Discussion

From: J. Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Tue Dec 30 2003 - 23:29:01 EST


Dear cytometry/cytomics colleagues:

2003 has had more ups than downs! As I usually do, I want 
to review the year from the perspective of our Purdue 
community who provide many resources for the cytometry 
community.

We completely rebuilt our web server hardware this year at 
a cost of about $30k. Yesterday, we did the audit of the 
entire years access to the PUCL server. It was totally 
overwhelmed with over 4 million successful requests for 
pages in 2003. The poor server delivered on average 441 
megabytes of data per day every day for the last 364 days! 
Over 200,000 distinct hosts were served by our server. Over 
100,000 distinct (i.e. different) files were delivered from our 
web archive. 2500 members currently participate in our 
cytometry discussion.  If anyone tells me that cytometry is 
not alive and well on planet earth, tell them they're 
dreaming! (only the Aussies who have seen "The Castle" 
will know what that means!)

The most accessed files are naturally #1 the email archive 
and #2 our education pages that currently contains over 
1000 powerpoint slides on flow cytometry, imaging and 
related areas. 
http://www.cyto.purdue.edu/flowcyt/educate/pptslide.htm

#3 are the CD-ROM pages that highlight our Purdue CD-
ROM series.
http://www.cyto.purdue.edu/flowcyt/cdseries.htm

 There is actually far too much information to be easily 
found and we are currently investigating ways to create a 
more useful database that might make the information much 
more accessible.

The most exciting thing by far this year has been your 
responses to our new Purdue Surveys. I closed the 
calibration survey after 384 people contributed. This tells a 
lot about the changing environment of how we see data 
analysis and how important standards and calibration are 
becoming (finally!!). 

The "Future of Cytometry" survey is still open and 530 
people have contributed so far. ( http://tinyurl.com/yq17 ). I 
am almost overwhelmed that so many people have taken 
the time to provide detailed comments on topics that clearly 
impact their professional lives. This survey is really exciting 
because it is revealing really important perspectives on how 
you see cytometry/cytomics. I am hoping that we will get 
another 1-200 respondents before we do a final analysis. I 
have already provided preliminary data to several colleages 
to get their initial opinions. I will post the entire survey and 
the analysis eventually for all to see. I hope that you will 
take the time to read what others are saying. I already 
mentioned that 96.7% of respondents say that they are 
optimistic about the future of cytometry.

This is not to say that things are great! I find it a great 
concern that a number of respondents did not know what 
ISAC was! Of 65 student respondents, only 13 were 
members of ISAC - since there are only currently 15 student 
members of ISAC  - I think you might see where my 
concerns are! "Houston, we have a problem"! 

What is on the horizon for 2004? Clearly the ISAC congress 
in Montpellier (www.isac-net.org) will be a highlight meeting. 
The area of CYTOMICS is not going away - indeed it was 
identifed by ISAC as an important developing area and it is. 
The cytomics sessions at the last ISAC congress set an 
important milestone (kilometer-stone just does not work 
does it!) I predict that we will all be talking more about 
cytomics than the more narrow perspective of cytometry 
over the next year. I think this is a good thing.  Perhaps 
2004 will see us rename our Purdue Cytometry service to 
Purdue Cytomics....in any case, I think that 2004 is going to 
be a year of change. I can tell you that we are changing our 
programs and directions at Purdue more than in any time in 
the past 15 years. Look forward to 2 new CD-ROMs in 2004 
- Cytometry #8 and Microscopy and Imaging #2..(we are 
looking for sponsors by the way)

Finally, if you want to know who to blame for talking the 
most on the list,.....here is the the 2003 top 20 villains in 
responses...

Howard Shapiro 71 (always gets first prize and justifiably 
so!)
J. Paul Robinson 46
Marty Bigos  36
Joanne Lannigan 28
Zbigniew Darzynkiewicz 27
William Telford 25
David Coder 21
Mario Roederer	20
Nebe-Von-Caron, G 20
Albert Tai 20
Simon Monard  20
Richard Haugland 19
Gerstein, Rachel 17
Prussin, Calman  16
Derek Davies 16
Fischer, Randy 16
David.C.McFarland@gsk.com 15
Frank Battye 15
Alice L. Givan 14
Stetler-Stevenson, Maryalice 13

thats it for 2003. 
May your flow cells not clog, 
may your gates remain latched, 
may your compensation be propery Mario'd
and your data backed up before the crash
may your fluorescence not fade too fast
and your lenses only become slighly scratched
when your cytometer crashes, be glad it restarts
Don't drink too much on New Year's eve so you
all make it to 2004 mostly in one piece.

best wishes from our Purdue family who I want to 
acknowledge because they all contribute significantly to our 
program, our website and make all the CDs.

-Kathy Ragheb and Cheryl Holdman (our great cytometry 
technicians)
-Jenny Sturgis (our great image analysis technician)
-Gretchen Lawler (Editorial assistant for Current Protocols)
Bartek Rajwa and Gerald Gregori (our super postdocs)
Steve Kelley (our computer and networking command 
center)
and lots of students....

J. Paul Robinson
Professor and Director






J.Paul Robinson, PhD		 PH:(765)4940757
Professor of Immunopharmacology
Professor of Biomedical Engineering  
Purdue University	   FAX:(765)4940517
EMAIL:jpr@flowcyt.cyto.purdue.edu
WEB: http://www.cyto.purdue.edu   


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