From: Perfetto, Steve (NIH/VRC) (perfetto@mail.nih.gov)
Date: Mon Dec 22 2003 - 22:00:27 EST
Andy, We tested this extensively with earlier versions of the DiVa software and in fact used the FlowJo program to determine the percentages to use in the DiVa program. In both of these cases over many test samples using 10 to 12 color analysis the percentages were identical. While the data can be transmitted as a FSC file, the version of the FSC conversion is the key. You must use FCS 3.0 or higher conversion. However, the most ideal conversion is the experiment export. Check the version of DiVa software you are using, I believe the earlier versions did not support these export forms. Stephen P. Perfetto, MS.,MT. (ASCP)Manager, Core Flow Cytometry FacilityVaccine Research Center, NIH40 Convent Dr., Room 5507Bethesda, MD 20892-3015email: sperfetto@nih.govPhone: (301) 594-8659 > ---------- > From: Andy Johnson > Sent: Friday, December 19, 2003 3:01 PM > To: Cytometry Mailing List > Subject: DiVa and FlowJo issue > > Hello > > Can I just ask if anybody else is having trouble converting DiVa > files into similar FlowJo files ? > > There appears to be no problem when files are not compensated in > DiVa. The problem occurs when DiVa compensated files are read in > FlowJo resulting in "massive" over compensation of the data. > Unfortunately we have got little help from FlowJo other than try > their new version...which we have with similar results. > > We are looking at 8 colours plus, so is it just that the compensation > matrix of the DiVa and flowJo clash ? > > We could save uncompensated data in DiVa and look at that in > FlowJo...but for the price you pay, this should not have to be the > case ? Plus we are saving large files of rare events, so this would > > remove a large portion of our sorting sample. > > Any suggestions ? > > Andy > Merry Christmas > -- > Andy Johnson > FACS Facility Manager > Biomedical Research Centre > UBC > Vancouver > 604-822-7838 > >
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