From: J. Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Fri Dec 19 2003 - 17:23:53 EST
On 19 Dec 2003 at 7:02, Stewart, Judith wrote: >I have a user interested in looking at the oxidative process in brain tissue from rats. >Does anybody have any experience in using tissue for this assay and for flow on such >cells? >I've used the dihydrorhodamine assay on whole blood but I have no experience with >tissue. Thanks to all of you. We have done work on looking at the oxidative process in spinal cord "The increase of reactive oxygen species and their inhibition in an isolated guinea pig spinal cord compression model", Luo,J, Li, N., Robinson, J.Paul, & Shi, R., J Biomech Eng 124:214-222, 2002. "Detection of reactive oxygen species by flow cytometry after spinal chord injury" Luo, J. Li, N., Robinson, J. Paul, & Shi, R., Journal of Neuroscience Methods 120:105-112, 2002 We used the same dyes. I think you will see some solutions to the issues in these manucripts. regards J.Paul Robinson -- J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL: jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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