From: Nathan Regimbal (Nathan.Regimbal@biogenidec.com)
Date: Thu Dec 18 2003 - 16:07:47 EST
John, Here's a document with my take on the matter. Those who know everything about everything are certain to find a few things wrong with this if they take time away from penning new books to take a gander, but as a general guide to getting data I think this will do the trick for us normal people. It covers the basics. It says nothing about editing run-on sentences. At the time Purdue delivered this email to me, I was actually writing script for a cell cycle analysis training video (to add to the collection that'll be unleashed on the world's rookie flow users soon). Cool coincidence. Here's the doc for all to enjoy and evaluate -- hopefully it'll be useful for some. Sunny and 70 in December, Nate Nate Regimbal Biogen Idec, Inc. v 858.431.8293 f 858.431.8715 "Dr. John Waitumbi" <JWaitumbi@kisian.mimcom.net> 12/17/2003 11:55 PM Message Size: 5.1 KB To "Cytometry Mailing List" <cytometry@flowcyt.cyto.purdue.edu> cc Subject Re: DNA analysis I'm stuck at the basics. Can someone give me tips on setting up doublet discriminator on FACScan Lysis II or FACScalibur. I cannot see my PI stained cells on pulse width or pulse area (set in FL2). I've tried to up the amplificatiion gain etc but cannot see a single cell on the computer but can see pulses on the cytometer. John
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