From: Stewart, Judith (Judith_Stewart@URMC.Rochester.edu)
Date: Fri Dec 19 2003 - 07:02:20 EST
Holiday wishes to all my fellow flow users. I have a user interested in looking at the oxidative process in brain tissue from rats. Does anybody have any experience in using tissue for this assay and for flow on such cells. I've used the dihydrorhodamine assay on whole blood but I have no experience with tissue. Thanks to all of you. According to my user: "The idea would be to (excuse the gory details) cut the skull open and remove the various brain regions by scooping out and peeling away the structures. Homogenization would likely kill a good number of cells, so I am thinking that some form of gentle disaggregation would work better. The structure we are probably most interested in is olfactory bulb,but would also want to compare to other regions as well. As I said earlier, the main thing would probably be to find a way of identifying the cells that are producing the signal. However, the overall signal would also be of interest."
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