Re: DNA analysis

From: Dr. John Waitumbi (JWaitumbi@kisian.mimcom.net)
Date: Thu Dec 18 2003 - 02:55:26 EST

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I'm stuck at the basics. Can someone give me tips on setting up doublet discriminator on FACScan Lysis II or FACScalibur. I cannot see my PI stained cells on pulse width or pulse area (set in FL2). I've tried to up the amplificatiion gain etc but cannot see a single cell on the computer but can see pulses on the cytometer.

John

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