Re: Concentration of Brefeldin A in Golgiplug?

From: Charles Maris (cmaris@emory.edu)
Date: Wed Dec 17 2003 - 16:03:55 EST


Call BD technical help.  I had the same issue, although I don't 
remember what the concentration is off the top of my head.

-Charles Maris
On Wednesday, December 17, 2003, at 03:13 AM, Philipp Röntgen wrote:

> Dear all,
>
> i am writing my thesis on intracellular cytokine staining in monocytes.
> I was using Golgiplug 1µl/1ml according the protocol from BD for
> intracellular accumulation. I am interested in the proper concentration
> of Brefeldin A in the stock solution from BD. Does anybody know the
> concentration of Brefeldin A in Golgi Plug and of what reagents and
> concentrations Golgiplug consists?
>
> thx for the help
>
> Philipp Rƶntgen
> Dep. of cardio thorathic surgery
> Ernst Moritz Arndt Str. 8
> 06114  Halle
> Germany
>
>


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