From: Greg Perry (cytometry@creighton.edu)
Date: Thu Dec 18 2003 - 09:04:16 EST
We run bacteria nearly every day on a FACSCalibur. Works fine. Basically, run all scatter and fluorescence parameters in log. Let me know if you need more detail and good luck! - Greg Greg A. Perry, Ph.D. Technical Director Creighton University Flow Cytometry Core Facility > From: James Kirby <jekirby@bidmc.harvard.edu> > Date: Wed, 17 Dec 2003 10:50:24 -0500 > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: flow of microbiology > > Dear All, > I was interested in finding protocols for doing flow on bacteria to > enumerate bacterial numbers and determine viability. We have a BD > FACSCalibur. Is anyone using this particular cytometer for > bacterial analysis. What general settings are used for detecting > bacteria in assays? > > Thanks, > James > -- > James E. Kirby > Assistant Director, Clinical Microbiology > Beth Israel Deaconess Medical Center > Department of Pathology > 330 Brookline Avenue - Yamins 309 > Boston, MA 02215 > (617) 667-3648 (phone) > (617) 667-4533 (fax) > jekirby@bidmc.harvard.edu > > >
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