Re: flow of microbiology

From: Greg Perry (cytometry@creighton.edu)
Date: Thu Dec 18 2003 - 09:04:16 EST


We run bacteria nearly every day on a FACSCalibur.  Works fine.  Basically,
run all scatter and fluorescence parameters in log.  Let me know if you need
more detail and good luck!

 - Greg

Greg A. Perry, Ph.D.
Technical Director
Creighton University
Flow Cytometry Core Facility

> From: James Kirby <jekirby@bidmc.harvard.edu>
> Date: Wed, 17 Dec 2003 10:50:24 -0500
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: flow of microbiology
> 
> Dear All,
> I was interested in finding protocols for doing flow on bacteria to
> enumerate bacterial numbers and determine viability.	We have a BD
> FACSCalibur.	Is anyone using  this particular cytometer for
> bacterial analysis.  What general settings are used for detecting
> bacteria in assays?
> 
> Thanks,
> James
> -- 
> James E. Kirby
> Assistant Director, Clinical Microbiology
> Beth Israel Deaconess Medical Center
> Department of Pathology
> 330 Brookline Avenue - Yamins 309
> Boston, MA 02215
> (617) 667-3648 (phone)
> (617) 667-4533 (fax)
> jekirby@bidmc.harvard.edu
> 
> 
> 


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