From: Philipp Röntgen (ph.roentgen@gmx.net)
Date: Wed Dec 17 2003 - 03:13:20 EST
Dear all, i am writing my thesis on intracellular cytokine staining in monocytes. I was using Golgiplug 1µl/1ml according the protocol from BD for intracellular accumulation. I am interested in the proper concentration of Brefeldin A in the stock solution from BD. Does anybody know the concentration of Brefeldin A in Golgi Plug and of what reagents and concentrations Golgiplug consists? thx for the help Philipp Röntgen Dep. of cardio thorathic surgery Ernst Moritz Arndt Str. 8 06114 Halle Germany
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