Concentration of Brefeldin A in Golgiplug?

From: Philipp Röntgen (ph.roentgen@gmx.net)
Date: Wed Dec 17 2003 - 03:13:20 EST


Dear all,

i am writing my thesis on intracellular cytokine staining in monocytes.
I was using Golgiplug 1µl/1ml according the protocol from BD for
intracellular accumulation. I am interested in the proper concentration
of Brefeldin A in the stock solution from BD. Does anybody know the
concentration of Brefeldin A in Golgi Plug and of what reagents and
concentrations Golgiplug consists?

thx for the help 

Philipp Rƶntgen
Dep. of cardio thorathic surgery
Ernst Moritz Arndt Str. 8
06114  Halle
Germany


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