From: Ivey, John (John.Ivey@health.wa.gov.au)
Date: Tue Dec 16 2003 - 19:46:41 EST
Would anyone know the best way to denature PicoGreen? (see thread below) --------------------------- Query --------------------------- We wish to use a Gilson liquid handling system to pipette this PicoGreen into 96 well trays. The liquid handler has a metal probe and teflon tubing. After use we will flush the system with water. Over time is the dye likely to bind to the tubing? What is the best way to denature the dye? Is Picogreen sufficiently light sensitive that the small amount that might bind would be denatured by ambient light (fluorescent light tubes)? Could you recommend a washing solution to ensure complete removal/denaturation of the dye. Thanks ------------------------------- It is being used at an unspecified concentration (as outlined in the Molecular Probes dsDNA Quantitation kit product information leaflet). I have asked Molecular Probes, but they were not able to help. Thanks and Merry Christmas John John Ivey Principal Scientist Cell Biology Unit Department of Haematology Royal Perth Hospital GPO Box X2213 Perth 6847 AUSTRALIA Phone: 61 8 9224 2410 Fax: 61 8 9224 3449 --------------------------- Query --------------------------- We wish to use a Gilson liquid handling system to pipette this PicoGreen into 96 well trays. The liquid handler has a metal probe and teflon tubing. After use we will flush the system with water. Over time is the dye likely to bind to the tubing? What is the best way to denature the dye? Is Picogreen sufficiently light sensitive that the small amount that might bind would be denatured by ambient light (fluorescent tubes)? Could you recommend a washing solution to ensure complete removal/denaturation of the dye. Thanks -----Original Message----- From: Larry Yamaoka [mailto:larry.yamaoka@probes.com] Sent: 17 December 2003 07:36 To: cyto-inbox Subject: PicoGreen P7581 Dear Researcher: PicoGreen dsDNA quantitation reagent may bind to tubing over time. However, our scientists have not examined this question. I suggest contacting the company that makes your liquid handling equipment for their recommendations for cleaning. Thank you for your interest in Molecular Probes and its products. Please contact me if you need additional information. Sincerely, Larry Yamaoka Technical Assistance Representative Molecular Probes, Inc. Phone: (541) 335-0353 Fax: (541) 335-0238 larry.yamaoka@probes.com Please visit our web site: www.probes.com Country: Australia E-mail: john.ivey@health.wa.gov.au Subject: Catalog Number P7581 --------------------------- Query --------------------------- We wish to use a Gilson liquid handling system to pipette this reagent into 96 well trays. The liquid handler has a metal probe and teflon tubing. After use we will flush the system with water. Over time is the dye likely to bind to the tubing? What is the best way to denature the dye? Is Picogreen sufficiently light sensitive that the small amount that might bind would be denatured by ambient light (fluorescent tubes)? Could you recommend a washing solution to ensure complete removal/denaturation of the dye. Thanks
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:44:06 EST
