ISHAGE procedure for mice bone marrow?

From: mattiaqu@tin.it
Date: Tue Dec 16 2003 - 05:38:59 EST

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Dear all,
	    I'm using the ISHAGE procedure (CD45/CD34 staining plus side
and forward scatter evaluation) to count CD34+ cells in mouse bone marrow.

Are there any references that you know or can you tell me your experience
about using this procedure in mice?

Do you think that a similar procedure would also be applicable for others
stem cell markers such as c-kit or thy-1? 
Would it be reliable? 

Any advice will be appreciated

Best Regards,

MATTIA QUARTA M.D. 
Department of Medical and Surgical Sciencies
University of Padua
Via Giustiniani 2
35100, PADOVA
Italy
Phone: +39-049-8211873
fax: +39-049-8211884

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