From: Kundu, Mondira (mondira_kundu@uphs.upenn.edu)
Date: Mon Dec 08 2003 - 15:55:22 EST
I have a few questions on telomere length detection by flow fish... Why are Alexa Fluor 488 and Alexa Fluor 546 used instead of FITC and PE for cell surface staining when combining with the flow-fish protocol for telomere length measurement? (I assume that they are better able to withstand the treatment, so specifics would be useful). It seems that there is a significant correlation between telomere length and CML disease stage (Brummendorf et al Ann NY acad sci,2003 & Brummendorf et al Blood 2000) but that there is a lot of variability within the groups. What are thoughts on the potential clinical utility of this test? (it seems that it may be useful at diagnosis as another marker of the PH+ cells, in cases where there is a difference between PBLs and Ph- Tcells) Also, does anyone have a slide comparing different methods of telomere length detection to flow-fish that I could use? (I am a Pathology resident and I will be giving an "educational" talk to the department on use of flow-fish for telomere length and applications in CML). Thanks! Mondira Kundu, M.D., Ph.D.
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