Oxidative Burst for craig@upmc.edu

From: giaccaia@mail.ilww.it
Date: Fri Dec 05 2003 - 09:00:51 EST


Hi,
I tried to send you the answers about the oxidative burst, but the mails
came back.
So I try thi way.

First mail


Luigi, We have done hundreds of oxidative burst on patients that are being
screened for Chronic Granulomatous Disease. The normal range in our lab for
pediatrics is the same as the adult range. There is no difference between
the two groups.

We use something called a stimulation index. We place the unstimulated
cells
in channel ten (on a log scale). Then we run the stimulated and
unstimulated
samples. We divide the stimulated geometric mean by the unstimulated
geometric mean. The resulting number is the stimulation index or S.I. In
our
lab an S.I. greater than 115 is considered normal.

Maggie Brown



Margaret Brown, MT (ASCP)
Immunology Flow Cytometry Lab
Department of Laboratory Medicine
Bldg. 10 /Rm 2C410
Clinical Center, National Institutes of Health
Bethesda, MD 20892-1508

phone: 301-496-4879
FAX: 301-402-1884

Second mail

Contact thomas.nebe@ikc.ma.uni-heidelberg.de or look at
http://www.ma.uni-heidelberg.de/inst/ikc/ikc-fd-hema-immu.html My
brother has done a number of studies with good results. They actually
use it as a regular tool in the hospital

Regards

Gerhard


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