From: J. Paul Robinson (jpr@flowcyt.cyto.purdue.edu)
Date: Mon Nov 24 2003 - 22:38:55 EST
I thought I just answered this question....was I dreaming....? There is an enormous amount of information already in the archive. I understand it is difficult to extract this and we are working on this to improve the system. But it is important for new participants to use the search engines to find answers to questions that may have already been answered. regards paul robinson On 21 Nov 2003 at 16:15, Sabyasachi Biswas wrote: > Hi > > Does anyone know whether 2',7'-dichlorofluorescin diacetate (H2DCFDA) > photodecomposes? We are trying to detect H2O2 generation in lymphocytes but our > results are anomalous. Microscopic examination suggested that H2DCFDA decomposed > and fluoresced when excited with FITC filter in our fluorescence microscope. > However we couldn't find any reference for this phenomenon. Any information > regarding this will be very helpful. > > Thanks > > Sabyasachi Biswas > > J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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