2',7'-dichlorofluorescin diacetate photodecomposition

From: Sabyasachi Biswas (biswas.16@osu.edu)
Date: Fri Nov 21 2003 - 16:15:20 EST

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Hi

Does anyone know whether 2',7'-dichlorofluorescin diacetate (H2DCFDA) photodecomposes? We
are trying to detect H2O2 generation in lymphocytes but our results are anomalous.
Microscopic examination suggested that H2DCFDA decomposed and fluoresced when excited
with FITC filter in our fluorescence microscope. However we couldn't find any reference
for this phenomenon. Any information regarding this will be very helpful.

Thanks

Sabyasachi Biswas

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In reply to: Sabyasachi Biswas: "2',7'-dichlorofluorescin diacetate"
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