2',7'-dichlorofluorescin diacetate

From: Sabyasachi Biswas (biswas.16@osu.edu)
Date: Thu Nov 20 2003 - 16:52:02 EST

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Hi

Does anyone know whether 2',7'-dichlorofluorescin diacetate (H2DCFDA) photodecomposes? We
are trying to detect H2O2 generation in lymphocytes but our results are anomalous.
Microscopic examination suggested that H2DCFDA decomposed and fluoresced when excited
with FITC filter in our fluorescence microscope. However we couldn't find any reference
for this phenomenon. Any information regarding this will be very helpful.

Thanks

Sabyasachi Biswas

Next message: Bakul Dalal [Dr.] [VH]: "Re: Réf. : PNH Panels"
Previous message: William Telford: "Re: Fwd: UV-laser LSR II"
Next in thread: J. Paul Robinson: "Re: 2',7'-dichlorofluorescin diacetate; Response"
Reply: J. Paul Robinson: "Re: 2',7'-dichlorofluorescin diacetate; Response"
Maybe reply: Guenter Valet: "Re: 2',7'-dichlorofluorescin diacetate"
Reply: Sabyasachi Biswas: "2',7'-dichlorofluorescin diacetate photodecomposition"
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