From: McCoy, J. Philip (NIH/NHLBI) (mccoyj@NHLBI.NIH.GOV)
Date: Tue Nov 18 2003 - 16:09:23 EST
<?xml version="1.0" ?> I would use fluorochrome-labelled CD31 or CD146 and use fluorescence to separate the HUVEC microparticles from debris Phil J. Philip McCoy, Jr., Ph.D. Director of the Flow Cytometry Core Facility NHLBI - NIH Bldg 10, Rm 4A07 10 Center Dr., MSC 1357 Bethesda, MD 20892 -----Original Message----- From: Campbell, Hope [mailto:HMCampbell@bcsew.edu] Sent: Monday, November 17, 2003 5:43 PM To: cyto-inbox Subject: microparticles HI All- I have a question regarding the detection of microparticles on a LSRII or a FACScan. I have a user who is working with HUVEC microparticles(MPs). The size of the MP's are about 1 um in diameter. When we run his samples on the cytometer, FSC, SSC, and FL1 are in log. There is a broad population in the FSC, SSC dot plot, but I am unsure if I am gating debris with the MPs or if the sample was prepared incorrectly because there is no major histogram shift in FL1. I'm new to flow so my inexperience could be to blame. If anyone has worked with microparticles and can offer some cytometer set up suggestions or help me discern debris from MPs, I would appreciate the help. Thank you ---- Hope Campbell Blood Research Institute Blood Center of SE Wisconsin 414-937-3843 ***** CONFIDENTIALITY NOTICE ***** This transmission may contain confidential information. If you have received this transmission in error, please notify the sender immediately and destroy this material.
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:44:03 EST
