From: McCoy, J. Philip (NIH/NHLBI) (mccoyj@NHLBI.NIH.GOV)
Date: Thu Nov 06 2003 - 16:21:53 EST
You could try to use Hoechst to stain for the SP cells, which are stem cells. This method uses no MAbs. These will be very rare events in nonmobilized blood. Phil J. Philip McCoy, Jr., Ph.D. Director of the Flow Cytometry Core Facility NHLBI - NIH Bldg 10, Rm 4A07 10 Center Dr., MSC 1357 Bethesda, MD 20892 > -----Original Message----- > From: Strydom AV <12202800@sun.ac.za> [mailto:12202800@sun.ac.za] > Sent: Thursday, November 06, 2003 9:55 AM > To: Cytometry Mailing List > Subject: Stem cells through FACS without Ab...possible? > > > Hi > > Does anyone know whether I can put a buffycoat sample (from > which most of the RBC have > been removed) through a FACS to count the stem cells present. > I know it has been done > for WBC but will you be able to pick up the stem cells > without an Ab? Secondly if not, > then does anyone know where I can find CD45 anti-horse that > could take out the WBC from > the horse blood sample and then put it through the FACS to > count the stem cells....or of > any horse Ab for stem cells, specifically mesenchymal stem cells? > Kind regards > Aliki V. Strydom > (PhD Candidate/PhD Kandidaat) > Animal Biotechnology Laboratory / Dier Biotegnologie Laboratorium > Department of Animal Sciences / Departement Veekundige Wetenskappe > Stellenbosch University / Universiteit Stellenbosch > Private Bag X1, Matieland 7602, South Africa / Privaatsak X1, > Matieland 7602, Suid-Afrika Phone/Tel: +27 (0)21 808 3150 > Fax/Faks: +27 (0)21 808 3028 > Cellphone/Sel: +27 (0)82 689 1088 > E-mail/E-pos: 12202800@sun.ac.za / dan2000@mweb.co.za > Hasta la victoria siempre - Che Guevara > We will continue until we are successful - Animal Biotech Lab > >
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