From: McCoy, J. Philip (NIH/NHLBI) (mccoyj@NHLBI.NIH.GOV)
Date: Mon Oct 27 2003 - 17:30:50 EST
I would suggest that it depends on both the size of the cells you are analyzing (or sorting) and the size nozzle tip that you are using. Certainly you would not want to filter with a larger pore size than the opening of your nozzle (see one of Howard's laws). For lymphs, which are roughly 7 microns in diameter, we often use a 40 micron mesh if we sort with either 50 or 70 micron tips. We occasionally use 70 micron filters and seldom see clumping if we are using a 70 micron tip...but I wouldn't go to this pore size if using a smaller tip. Phil J. Philip McCoy, Jr., Ph.D. Director of the Flow Cytometry Core Facility NHLBI - NIH Bldg 10, Rm 4A07 10 Center Dr., MSC 1357 Bethesda, MD 20892 > -----Original Message----- > From: Beverly Barton [mailto:bartonbe@umdnj.edu] > Sent: Thursday, October 23, 2003 3:52 PM > To: Cytometry Mailing List > Subject: pore size of nylon mesh > > > Hello: > > Does any know what size in microns is the correct pore size to order > Nitex nylon for filtering out clumps? > > Thank you. > > > Beverly E. Barton, Ph.D. > Assistant Professor > Department of Surgery/Division of Urology > UMD-New Jersey Medical School > 185 S. Orange Avenue MSB G-519 > Newark, NJ 07103 > USA > > 973-972-0662 phone > 973-972-3892 facsimile > bartonbe@umdnj.edu e-mail > >
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