From: A Herman, Pathology & Microbiology (A.Herman@bristol.ac.uk)
Date: Thu Oct 09 2003 - 04:02:23 EST
Hi Regis, Our experience using a Vantage SE for sorting murine DCs is that a 100um nozzle and approx. 17 PSI works well. Sorts using 70 um and 34 PSI on our Vantage appear to result in activation of naive murine lung DCs. Yours, Andrew Herman --On Tuesday, October 7, 2003 17:24 +0200 Regis Josien <rjosien@nantes.inserm.fr> wrote: > Dear flowers, > > We want to sort rat spleen and lymph node fresh DC on our FACS Aria. For > those of you who have experience in sorting rat or murine DC, could you > let me know the limit of pressure your cells tolerate. Thanks. > > -- > Regis Josien, MD, Ph.D > Institut de Transplantation et de Recherche en Transplantation > INSERM U437 > 30 bd Jean Monnet > 44093 Nantes Cedex 1 > France > tel : +33-240-087-413 > sec: +33-240-087-410 > fax: +33-240-087-411 > email : rjosien@nantes.inserm.fr > rjosien@chu-nantes.fr > ---------------------- A Herman, Pathology & Microbiology A.Herman@bristol.ac.uk
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:43:58 EST
