From: SIMONE BOER (SIMONEB@BarwonHealth.org.au)
Date: Tue Oct 07 2003 - 21:02:17 EST
> > Hi Guys > I wrote to you about 6 weeks ago asking for advice in regard to using > Fluo-3 and measuring the calcium response in platelets when stimulated. I > have finally gotten this assay to work. I found that the Fluo/DMSO mixture > was activating the platelets shortly after incubation. Thus, when I came > to stimulate the sample with my agonist, the platelets were already > activated and consequently were not responding upon stimulation. I just > want to say thankyou for all your help in helping me solve this problem. I > do have one more problem, which I would appreciate any ideas: > > I have found that in many of my samples I can produce a five-fold increase > in flouresence upon stimulation. However, in some cases, once the > response has ceased, the flourescence drops to levels below that of > baseline. > > Please view the attachment of my results. I have read much literature > regarding Fluo-3 and the calcium flux in cells and am unable to explain > what is occurring. I was wondering if this is a result of the Fluo-3, > somehow being able to leave the cell. After the fluo-3 has emitted > fluorescence, does it stop. Or does the calcium leave the cell after > influx (thus the fluo-3 follows out )? > > Any suggestions on what may be occuring would be gratelfully appreciated. > Thanking you for your time > Simone Boer > > > <<testflow.ppt>> MAIL IS CONFIDENTIAL. If you have received this e-mail in error, please notify us by return e-mail and delete the document. If you are not the intended recipient you are hereby notified that any disclosure, copying, distribution or taking any action in reliance on the contents of this information is strictly prohibited and may be unlawful. Barwon Health is not liable for the proper and complete transmission of the information contained in this communication or for any delay in its receipt.
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