From: Martin Poirier (martin_poirier@videotron.ca)
Date: Fri Sep 19 2003 - 19:41:39 EST
Hi everyone, I'm doing basic CD3/4/8a/45RA/161a staining on rat blood, and as far as I remember, I have always seen a population with High FSC/Low SSC on the light scatter dot plot. I wonder what this population might be as I was told many different things like LGL, stem cells, atypic lymphocytes, and I don't know who to believe I cannot find any info on the internet regarting that population (exhaustive info about light scatter properties of cells on FSC/SSC dot plots is hard to come by). I drew a picture to show the approximate appearance of the dot plots I see. Note that my drawing is in no way an exact representation of what I see. Unfortunately, I forgot the real data picture at my office, and I didn't want to wait until Monday to get an answer to this, hence the reason why I drew the mockup plot myself. The population I'm talking about is circled in red on my drawing, and is in the 800 range on the FSC axis. I see this with Sprague-Dawley (8 to 10 weeks old) and Fisher (6 to 12 weeks old) rats. Didn't try other strains. Any input is welcome, Martin
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